美国亚利桑那大学分子生物学方向博后职位招聘

Job Description

The Position Summary:
Department of Physiology and the research laboratory of Dr. Ayako Makino are seeking a highly-motivated Postdoctoral Fellow (Molecular Biologist). Our area of research focuses on the vascular complication in diabetes, in particular mitochondrial function, calcium handling in the endoplasmic reticulum, and cell-cell communication in endothelial and smooth muscle cells. The incumbent must have skills to conduct molecular biological experiments (cloning, development and application of Adenoviral- and Adeno-associated viral vectors, Western Blot, site-directed mutagenesis, qRT-PCR, single-cell RT-PCR).

Duties and Responsibilities:
• Perform molecular biological experiments (i.e., cloning, development and application of Adenoviral- and Adeno-associated viral vectors, Western Blot, site-directed mutagenesis, qRT-PCR, single-cell RT-PCR)
• Isolate vascular endothelial/smooth muscle cells from mouse heart and lung
• Conduct fluorescence microscopy experiments to measure Ca2+ and ROS concentration in cytoplasm and mitochondria
• Data acquisition and analysis, grant and manuscript preparation, and presentation of findings at weekly lab meetings
• Coordinate with other investigators for lab research including maintaining the function of lab equipment, managing the lab research record (including methodological approaches and animal protocols), and maintaining transgenic mouse colony

Knowledge, Skills, & Abilities:
• Skill in conducting cloning, generation of Adv and AAV, site-directed mutagenesis, PCR, qPCR, and WB
• Skills of animal handling and management of mouse colony
• Aseptic techniques
• Cell culture
• Ability to write a draft of manuscript and edit the content
• Knowledge of the principles and techniques of the subject discipline

Arizona Board of Regents Minimum Qualifications:
Successful candidate must have a Ph.D. in the biomedical sciences and at least two-year work experience in a laboratory are required.