Postdoctoral Associate- Neurobiology/Stem Cell - Stanley Center : Cambridge, MA, United States

A highly motivated and detail-oriented postdoctoral research associate is sought to lead cutting-edge research in imaging-based phenotypic profiling of iPSC-derived neuronal cells. The postdoctoral researcher will work jointly between the laboratories of Professors Mark Bathe (Department of Biological Engineering, MIT, and the Stanley Center for Psychiatric Research at the Broad Institute of MIT & Harvard) and Lee Rubin (Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, the Stanley Center for Psychiatric Research at the Broad Institute).

We are applying a highly multiplexed fluorescence-imaging platform to characterize protein and transcript levels and localizations in situ in human iPSC-derived neuronal cultures and spheroids. These imaging-based phenotypic datasets are being used to characterize in situ cellular differentiation state and neuronal structure to inform gene editing and differentiation protocols for modeling human diseases including schizophrenia and autism.

The successful candidate will work closely with a highly collaborative and energetic team of results- and application-focused engineers and scientists at MIT, Harvard, and Broad to perform antibody/nanobody/aptamer- and cDNA-labeling of target proteins and RNAs in iPSC-derived neuronal samples together with advanced fluorescence imaging using microfluidics-based exchange of imaging probes.

REQUIREMENTS

A Ph.D. in neurobiology, biology, biophysics, biochemistry, bioengineering or related field is required. Extensive experience in neuronal cell culture, immunofluorescence of cell cultures and tissues, advanced fluorescence imaging including some combination of confocal/TIRF/SPI microscopy. Proficiency in basic biochemical assays (chemical conjugation, SDS page, Western Blotting, etc.), and use of microfluidics is sought. Strong interpersonal, communication, and scientific skills are essential.