丹麦哥本哈根大学膜蛋白结构生物学方向研究职位招聘

丹麦哥本哈根大学膜蛋白结构生物学方向研究职位招聘

Job Description

Research area and project description

Our sensation of taste influences our dietary choices and alert us about harmful food, but the underlying molecular mechanisms taste sensations are poorly understood. The successful postdoc applicant will employ single- particle cryo-EM in combination with biochemical, pharmacological and cellular techniques to study the structure, dynamics and function of eukaryotic membrane-embedded proteins involved in taste signaling. The outcome of the project will yield unprecedented molecular insight into taste perception and delineate the therapeutic and diagnostic potential of an entire family of proteins.

The project is funded by a grant from the Novo Nordisk Foundation.

Project focus areas

The specific aim of the project is to study the structure, dynamics and function of eukaryotic membrane-embedded proteins involved in taste signaling. Practically, you will design and clone targets and express and purify well- behaved constructs to subject them to single-particle cryo-EM and optical analytical techniques to characterize protein-ligand and protein-lipid interactions. You will also develop and apply cell-based assays for further functional characterization. The position offers a mixture of techniques, and you are expected to steer the project in the required direction. You will work in a highly collaborative environment and are expected to participate in mentoring PhD students and other junior researchers in the lab.

Your qualifications

Experience within membrane protein structural biology is an advantage, but not a requirement. The formal requirements of the successful applicant are:

A PhD in structural biology, biophysics or biochemistry.

An excellent scientific track record, including a first-author publication within the field of structural biology, biophysics or biochemistry.

Experience with recombinant DNA techniques and cloning.

Experience with protein expression and purification.

Experience in data management and reporting.

Demonstrated ability to quickly grasp and employ new methods and tools.

An ability to work independently and as part of a team.

Proficiency in spoken and written English, with good interpersonal communication skills.

High level of motivation, responsibility, and ability to work in a structured manner.

As criteria for the assessment of your qualifications, emphasis will be laid on publications and relevant research experience. In addition to the formal requirements above, we are looking for a candidate that has experience within or strong qualifications for the following areas:

Membrane protein expression and purification.

Membrane protein-ligand binding assays.

Cryo-EM, X-ray crystallography and/or NMR.

Cryo-EM data processing and/or protein modelling.

Basic programming (MatLab and/or Python).