当前位置:知识人网 >> 国外博士后招聘 >> 正文内容

美国德州农工大学细菌蛋白出口单分子成像方向博士后
文章来源:知识人网整理       更新时间:2017年10月10日

Postdoctoral Fellow – Single Molecule Imaging of Bacterial Protein Export

A postdoctoral fellow is sought to decipher the mechanism of protein export by the bacterial Tat protein transport system using single molecule fluorescence (SMF) microscopy and single particle tracking approaches.  The focus will be on testing our recently proposed Hairpin-Hinge Model, which postulates a molecular mechanism whereby signal peptide conformational changes are intimately connected with pore assembly and cargo transport through the translocation pore.

Candidates with experience and/or interest in single molecule fluorescence microscopy, image analysis, adherent vesicles, protein translocation systems, MATLAB programming, and in vitro biochemistry and biophysics are strongly encouraged to apply.  A strong background and training in quantitative biology, with PhD emphasis ranging from physics to cell biology, is expected.  An interest in method development is essential.  The ideal candidate will be highly-motivated, and will be able to work independently.  The successful applicant will purify his/her own reagents, perform the single molecule experiments, and develop algorithms to analyze the results.

 

The Tat Protein Transport Project:

The twin-arginine translocation (Tat) machinery translocates folded proteins across the thylakoid membrane of plants and the cytoplasmic membrane of bacteria.  Using an efficient in vitro protein translocation assay, the Escherichia coli Tat system is amenable to characterization by a range of in vitro biochemical and biophysical assays.  The successful candidate will characterize the sub-steps of Tat transport using single molecule fluorescence microscopy methods.  A major focus will be to use single molecule fluorescence energy transfer to monitor conformational changes.  In addition to single molecule fluorescence microscopy, the project provides training in molecular biology, protein chemistry, membrane proteins, and image analysis.

扫一扫知识人网官方微信订阅号,关注更多学术资讯、博士后招聘信息。
国外博士后、访问学者申请免费专业评估,专业顾问在线答疑解惑。

快速申请国外博士后(免费评估)


点击这里快速申请国外博士后

点击这里快速申请国外访问学者

知识人网是专职国外博士后访问学者申请的服务机构,获评“北京诚信服务示范单位”。
自2004年起,累计十余年申请经验,以大量名校申请成功案例、及全球三万余所院校导师资源为基石,提供优质的申请咨询服务。
400电话:400-060-0581,欢迎您随时来电咨询!